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| Fonseca,Isabela; Silva,Priscila Vendramini; Lange,Carla Christine; Guimarães,Marta F.M.; Weller,Mayara Morena Del Cambre Amaral; Sousa,Katiene Régia Silva; Lopes,Paulo Sávio; Guimarães,José Domingos; Guimarães,Simone E.F.. |
| In order to characterize the expression of genes associated with immune response mechanisms to mastitis, we quantified the relative expression of the IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF-α genes in milk cells of healthy cows and cows with clinical mastitis. Total RNA was extracted from milk cells of six Black and White Holstein (BW) cows and six Gyr cows, including three animals with and three without mastitis per breed. Gene expression was analyzed by real-time PCR. IL-10 gene expression was higher in the group of BW and Gyr cows with mastitis compared to animals free of infection from both breeds (p < 0.05). It was also higher in BW Holstein animals with clinical mastitis (p < 0.001), but it was not significant when Gyr cows with and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytokine; Gene expression; Immune response; Real-time PCR. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400018 |
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